Human UBE2I ELISA Kit Size:96T Pipette 500 μL of the

Pipette 500 μL of the 16 ng/mL standard working solution into the first EP tube and mix thoroughly to make an 8 ng/mL standard working solution

allowing the rat Ab1-40 protein in the standard or sample to bind to the anti-rat Ab1-40 antibody immobilized on the microplate

mediates cell adhesion via alphavbeta3 integrin

centrifuge the prepared homogenate at 5000 × g for 5-10 minutes

Proto-oncogene c-Fos

Human UBE2I ELISA Kit Size:96T Pipette 500 μL of theProduct Specification Usage Self prepared test equipment required for the experiment: 1. Microplate reader (450nm) 2. High precision sampler and gun tip: 0. 5 10uL, 5 50uL, 20 200uL, 200 1000uL 3. 37 incubator 4. Distilled water or deionized water Sample handling and requirements: Tissue homogenate: Wash the tissue with pre cooled PBS (0. 01 M, pH = 7. 4) to remove residual blood (lysed red blood cells in the homogenate will affect the measurement