Human TGF-b2 ELISA Kit IHC Pathology Antibody Problem Description Possible cause Corresponding

Problem Description Possible cause Corresponding countermeasures Poor scaling curve Incorrect dilution of standard Ensure that the standard is dissolved and diluted according to the recommended method Inaccurate pipetting Calibrate the pipette periodically and check tip tightness Evaporation of the reaction solution Enzyme-labeled plate is sealed with a sealing membrane Incomplete plate washing Sufficient number of washes and addition of sufficient amount of washing liquid Foreign matter at the bottom of the hole Clean bottom of plate before reading Weak or colorless chromogenic Incubation time is not enough Ensure incubation time Incorrect incubation temperature Incubate at recommended temperature Insufficient reagent volume addition Inspect the pipette and follow the procedure exactly Incorrect dilution Test Reagent Dilution Step Enzyme conjugate inactivation Mixed Enzyme Conjugate and Substrate

10000rpm Centrifugation 10min

and cytokine receptor signaling

This cluster includes several genes involved in regulating immune responses

81 -500 pg/ml

Human TGF-b2 ELISA Kit IHC Pathology Antibody Problem Description Possible cause CorrespondingProduct Specification protein TGF b2 Usage Self prepared test equipment required for the experiment: 1 , plate reader ( 450nm 2 , high precision sampler and gun head: 0. 5 10uL 5 50uL 20 200uL 200 1000uL 3 37 Incubator 4 Distilled water or deionized water Sample handling and requirements: 1 The detection range of the kit is not equal to the concentration range of the substance to be tested in the sample. It is recommended to estimate the concentration