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Provides the most suitable carrier of foreign gene using particle bombardment
• Add 3 ml ice cold RIPA buffer to cell monolayer (or approximately 2–5 x 107 suspension cells in flask) and incubate at 4° C for 10 minutes
EMM-Glucose-CaCl2-MgCl2 Medium
It is simple to prepare
If you are interested in this same great product but as a fine powder for filtration
Calcium Chloride Anhydrous Size:1 kg Provides the most suitable carrierCalcium Chloride, anhydrous is a reagent used in tissue culture media and commonly used for competent cell preparation. Calcium chloride was used during the isolation of total glucan from hydrolyzed barley powders. Calcium Chloride also acts as a cross linker in the preparation of Aloe vera pectin gels.
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